VEGFA Kit ELISA

N° du produit ABIN8126240

Kit ELISA VEGFA Humain, Colorimetric test pour la quantification de Humain VEGFA.

Aperçu rapide pour VEGFA Kit ELISA (ABIN8126240)

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit VEGFA Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: This assay employs an antibody specific for Anti-human VEGF coated on a 96-well plate.

Analytical Method: Quantitative

Sensibilité: 9.0 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human VEGF concentrations in serum, plasma.  Detection Range: 31.2 - 2000 pg/mL Sensitivity: < 9.0 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.    

Restrictions: For Research Use only

Stockage

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Autre désignation: VEGF (Vascular Endothelial Cell Growth Factor)

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling