IL-13 Kit ELISA

N° du produit ABIN8126245

Le kit ELISA IL-13 (ABIN8126245) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris IL-13.

Aperçu rapide pour IL-13 Kit ELISA (ABIN8126245)

Antigène: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit IL-13 Kit ELISA

Seuil minimal de détection: 31.25 pg/mL

Fonction: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensibilité: 18.75 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-13 concentrations in serum, plasma.  Detection Range: 31.25 - 2000 pg/mL Sensitivity: < 18.75 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-13 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-13 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader, and then the concentration of IL-13 can be calculated.  

Restrictions: For Research Use only

Stockage

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: IL-13 (IL13) (Interleukin 13 (IL13))

Autre désignation: IL-13 (Interleukin 13)

Pathways: Signalistation JAK/STAT, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport