HSP27 Kit ELISA

N° du produit ABIN921115

Détail du produit HSP27 Kit ELISA

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Épitope: AA 2-205

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HSP27

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: T2-K205

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <5pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: T2-K205

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the small heat shock protein (HSP20) family.

Tissue Specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. .

Plaque: Pre-coated

Protocole: human HSP27 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HSP27 has been precoated onto 96-well plates. Standards(E.coli, T2-K205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HSP27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HSP27 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human HSP27 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human HSP27 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 256, Standard deviation: 10.75, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 1369, Standard deviation: 73.9, CV(%): 5.4
• Sample 3: n=16, Mean(pg/ml): 2628, Standard deviation: 144.5, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 279, Standard deviation: 17.6, CV(%): 6.3
• Sample 2: n=24, Mean(pg/ml): 1406, Standard deviation: 105.45, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 2635, Standard deviation: 213.4, CV(%): 8.1

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour HSP27 Kit ELISA (ABIN921115)

Ünver, Deveci, Kırkıl, Telo, Kaman, Kuluöztürk: "Serum Heat Shock Protein Levels and the Relationship of Heat Shock Proteins with Various Parameters in Chronic Obstructive Pulmonary Disease Patients." dans: Turkish thoracic journal, Vol. 17, Issue 4, pp. 153-159, (2018) (PubMed).

Détail du antigène

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSPB1 (HSPB1 Produits)

Synonymes: CMT2F Kit ELISA, HMN2B Kit ELISA, HS.76067 Kit ELISA, HSP27 Kit ELISA, HSP28 Kit ELISA, Hsp25 Kit ELISA, SRP27 Kit ELISA, 27kDa Kit ELISA, Hsp27 Kit ELISA, cb153 Kit ELISA, cb660 Kit ELISA, hsp1 Kit ELISA, hsp25 Kit ELISA, hsp27 Kit ELISA, id:ibd2821 Kit ELISA, sb:cb660 Kit ELISA, zgc:103437 Kit ELISA, HSPB1 Kit ELISA, cmt2f Kit ELISA, hs.76067 Kit ELISA, hsp28 Kit ELISA, heat shock protein family B (small) member 1 Kit ELISA, heat shock protein 1 Kit ELISA, heat shock protein, alpha-crystallin-related, 1 Kit ELISA, heat shock protein family B (small) member 1 L homeolog Kit ELISA, HSPB1 Kit ELISA, Hspb1 Kit ELISA, hspb1 Kit ELISA, hspb1.L Kit ELISA

Sujet:

Protein Function: Involved in stress resistance and actin organization.

Background: Hsp27 is a chaperone of the sHsp(small heat shock protein) group among ubiquitin, alpha-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7 kDa.

Synonyms: Heat shock protein beta-1,HspB1,28 kDa heat shock protein,Estrogen-regulated 24 kDa protein,Heat shock 27 kDa protein,HSP 27,Stress-responsive protein 27,SRP27,HSPB1,HSP27, HSP28,

Full Gene Name: Heat shock protein beta-1

Cellular Localisation: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

ID gène: 3315

UniProt: P04792

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling