IL-13 Kit ELISA

N° du produit ABIN921119

Détail du produit IL-13 Kit ELISA

Antigène: IL-13 (IL13) (Interleukin 13 (IL13))

Épitope: AA 19-131

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-13

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: T19-H131

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: T19-H131

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: rat IL-13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-13 has been precoated onto 96-well plates. Standards(E.coli, T19-H131) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-13 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat IL-13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IL-13 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 174, Standard deviation: 7.66, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 396, Standard deviation: 24.95, CV(%): 6.3
• Sample 3: n=16, Mean(pg/ml): 648, Standard deviation: 35, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 284, Standard deviation: 14.77, CV(%): 5.2
• Sample 2: n=24, Mean(pg/ml): 520, Standard deviation: 39, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 706, Standard deviation: 46.6, CV(%): 6.6

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL-13 Kit ELISA (ABIN921119)

Song, Li, Liang, Dai, Shen, Gong, Wang, Hu, Wang: "Low-level laser facilitates alternatively activated macrophage/microglia polarization and promotes functional recovery after crush spinal cord injury in rats." dans: Scientific reports, Vol. 7, Issue 1, pp. 620, (2017) (PubMed).

Huang, Ng, Chen, Lin, Tang, Cheng, Yang, Jiang: "Inhibition of TRPM7 channels reduces degranulation and release of cytokines in rat bone marrow-derived mast cells." dans: International journal of molecular sciences, Vol. 15, Issue 7, pp. 11817-31, (2015) (PubMed).

Détail du antigène

Antigène: IL-13 (IL13) (Interleukin 13 (IL13))

Autre désignation: IL13 (IL13 Produits)

Synonymes: IL13 Kit ELISA, IL-13 Kit ELISA, P600 Kit ELISA, Il-13 Kit ELISA, L13 Kit ELISA, interleukin 13 Kit ELISA, IL13 Kit ELISA, Il13 Kit ELISA

Sujet:

Protein Function: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses (By similarity). .

Background: Interleukin 13(IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2(Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor(IL-4Ralpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6(STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. Physical maps of the 5q23-q31 region show the following order: cen--IL3--CSF2--IL13--IL4--IL5--tel, with IL13 particularly close to IL4.

Synonyms: Interleukin-13,IL-13,T-cell activation protein P600,Il13,Il-13,

Full Gene Name: Interleukin-13

Cellular Localisation: Secreted.

ID gène: 116553

UniProt: P42203

Pathways: Signalistation JAK/STAT, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport