Lactate Dehydrogenase A Kit ELISA

N° du produit ABIN955920

Détail du produit Lactate Dehydrogenase A Kit ELISA

Antigène: Lactate Dehydrogenase A (LDHA)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 4.69-300 U/L

Seuil minimal de détection: 4.69 U/L

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of human LDHA in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of human LDHA. No significant cross-reactivity or interference was observed.

Sensibilité: The minimum detectable dose of human LDHA is typically less than 1.9 U/L. The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations.

Attributs du produit: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LDHA. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LDHA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LDHA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LDHA in the samples is then determined by comparing the O.D. of the samples to the calibration curve.

Ingrédients: Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x).

Matériel non inclus: 1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Commentaires:

The calibration curve concentrations used for the ELISA's were 300 U/L, 150 U/L, 75 U/L, 37.5 U/L, 18.75 U/L, 9.38 U/L, 4.69 U/L.

Plaque: Pre-coated

Calcul des résultats:

Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a calibration curve by plotting the mean absorbance for each calibrator on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the LDHA concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. It is recommended to use some related software to do this calculation. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.

Date de péremption: The expiry date is stated on the label.

Détail du antigène

Antigène: Lactate Dehydrogenase A (LDHA)

Autre désignation: LDH-A (LDHA Produits)

Synonymes: GSD11 Kit ELISA, LDH1 Kit ELISA, LDHM Kit ELISA, LDH-M Kit ELISA, ldh-h Kit ELISA, ldha Kit ELISA, ldha-B Kit ELISA, ldhbb Kit ELISA, trg-5 Kit ELISA, Ldh1 Kit ELISA, Ldhm Kit ELISA, l7R2 Kit ELISA, gsd11 Kit ELISA, ldh-m Kit ELISA, ldh1 Kit ELISA, ldhm Kit ELISA, pig19 Kit ELISA, ldha1 Kit ELISA, ldhab Kit ELISA, LDH-A Kit ELISA, LDHC Kit ELISA, lactate dehydrogenase A Kit ELISA, lactate dehydrogenase B L homeolog Kit ELISA, lactate dehydrogenase A4 Kit ELISA, lactate dehydrogenase A L homeolog Kit ELISA, L-lactate dehydrogenase A chain Kit ELISA, LDHA Kit ELISA, ldhb.L Kit ELISA, Ldha Kit ELISA, ldha Kit ELISA, ldha.L Kit ELISA, LOC100713875 Kit ELISA

Pathways: L'effet Warburg