PLG Kit ELISA

N° du produit ABIN956135

Détail du produit PLG Kit ELISA

Antigène: PLG (Plasminogen (PLG))

Reactivité: Porc

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: The Pig Plasminogen ELISA is a highly sensitive two-site enzyme-linked immunoassay (ELISA) for the quantitative determination of Plasminogen in pig biological fluids.

Analytical Method: Quantitative

Attributs du produit: In this assay the Plasminogen present in the sample reacts with the anti-Plasminogen antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Plasminogen antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Plasminogen. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Plasminogen in the sample tested, thus, the absorbance, at 450 nm, is a measure of the concentration of Plasminogen in the test sample. The quantity of Plasminogen in the test sample can be interpolated from the calibration curve constructed from the calibrators, and corrected for sample dilution.

Ingrédients: 1. Diluent Concentrate: One bottle containing 50 mL of a 5X concentrated diluent running buffer.
2. Wash Solution Concentrate: One bottle containing 50 mL of a 20X concentrated wash solution.
3. Enzyme-Antibody Conjugate Concentrate: One vial containing 150 µL of a 100X concentrated affinity-purified anti-pig Plasminogen antibody conjugated with HRP in a stabilizing buffer.
4. TMB Substrate Solution: One vial containing 12 mL of TMB and hydrogen peroxide in citric acid buffer at pH 3.3.
5. Stop Solution: One vial containing 12 mL of 0.3 M sulfuric acid. WARNING: Avoid contact with skin.
6. Microtiter Plate: Twelve removable eight-well strips in well holder frame. Wells are coated with affinity-purified anti-pig Plasminogen.
7. Pig Plasminogen Calibrator: One vial containing a lyophilized Pig Plasminogen Calibrator.

Matériel non inclus: Test tubes
Precision pipette (2 µL to 200 µL) for making and dispensing dilutions.
Microplate washer/aspirator
Distilled or de-ionized H2O Microplate reader
Assorted glassware for the preparation of reagents and buffer solutions
Timer
Vortex mixer

Information d'application

Plaque: Pre-coated

Préparation des réactifs:

1. Diluent Concentrate: The Diluent solution supplied is a 5X concentrate and must be diluted 1:5 with distilled or de-ionized water.
   2. Wash Solution Concentrate: The Wash Solution supplied is a 20X concentrate and must be diluted 1:20 with distilled or de-ionized water. Crystal formation in the concentrate is not uncommon when storage temperatures are low. Warming of the concentrate to 30-35°C before dilution can dissolve crystals.
   3. Enzyme-Antibody Conjugate Concentrate: Calculate the required amount of working conjugate solution for each microtiter plate test strip by adding 10 µL Enzyme-Antibody Conjugate to 990 µL of 1X Diluent for each test strip to be used for testing. Mix uniformly, but gently. Avoid foaming.
   4. TMB Substrate Solution: Ready to use as supplied.
   5. Stop Solution: Ready to use as supplied.
   6. Microtiter Plate: Ready to use as supplied. Unseal Microtiter Pouch and remove plate from pouch. Remove all strips and wells that will not be used in the assay and place back in pouch and re-seal along with desiccant.
   7. Pig Plasminogen Calibrator: Add 1.0 mL of distilled or de-ionized water to the lyophilized Pig Plasminogen Calibrator and mix gently until dissolved. The calibrator is now at a concentration of 19.06 µg/mL (reconstituted calibrator should be aliquoted and frozen if future use is intended). Pig Plasminogen calibrators need to be prepared immediately prior to use (see the following chart). Mix well between each step. Avoid foaming.

Calcul des résultats:

1. Subtract the average background value from the test values for each sample.
   2. Using the results observed for the calibrators construct a calibration curve. The appropriate curve fit is that of a four- parameter logistics curve. A second order polynomial (quadratic) or other curve fits may also be used.
   3. Interpolate test sample values from the calibration curve. Correct for serum dilution factor to arrive at Plasminogen concentration in original sample.
   
   1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the information contained in the package insert instructions and with adherence to good laboratory practice.
   2. Factors that might affect the performance of the assay include proper instrument function, cleanliness of glassware, quality of distilled or de-ionized water, washing thoroughly and accuracy of reagent and sample pipettings, washing technique, incubation time or temperature.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 1. Complete Kit: The expiration date for the kit is stated on the outer label. The recommended storage temperature is 4°C. Note: See long term storage recommendations below for the Pig Plasminogen Calibrator.
2. Diluent: The 5X Diluent Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4°C.
3. Wash Solution: The 20X Wash Solution Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (RT, 16-25°C) or at 4°C.
4. Enzyme-Antibody Conjugate: Undiluted horseradish peroxidase anti-Plasminogen conjugate should be stored at 4°C and diluted immediately prior to use. The working conjugate solution is stable for up to eight hours.
5. TMB Substrate Solution: The TMB Substrate Solution should be stored at 4°C and is stable until the expiration date.
6. Stop Solution: The Stop Solution should be stored at 4°C and is stable until the expiration date.
7. Microtiter Plate: Anti-pig Plasminogen coated wells are stable until the expiration date and should be stored at 4°C in the sealed foil pouch with a desiccant pack.
8. Pig Plasminogen Calibrator: The lyophilized Pig Plasminogen Calibrator should be stored at 4°C or frozen until reconstituted. The reconstituted calibrator should be aliquoted and stored frozen (avoid multiple freeze-thaw cycles). The working calibrator solutions should be prepared immediately prior to use and are stable for up to eight hours.

Date de péremption: The expiry date is stated on the label.

Détail du antigène

Antigène: PLG (Plasminogen (PLG))

Autre désignation: Plasminogen (PLG Produits)

Synonymes: wu:fb70e09 Kit ELISA, PLG Kit ELISA, LPA Kit ELISA, plg Kit ELISA, Ab1-346 Kit ELISA, AI649309 Kit ELISA, Pg Kit ELISA, plasminogen Kit ELISA, plg Kit ELISA, PLG Kit ELISA, Plg Kit ELISA

Sujet: Plasminogen (PMG) is a glycoprotein produced by the liver. It is the precursor for plasmin, which targets fibrin in the process of dissolution of fibrin blood clots. Plasminogen is present in plasma and most extravascular fluids. The important role of plasminogen in the fibrinolytic system makes it an interesting marker for various diseases.

Pathways: Système du Complément, Lipid Metabolism