IGF1 Protéine

N° du produit ABIN1111672

Protéine Recombinant IGF1 exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 1 publication.

Aperçu rapide pour IGF1 Protéine (ABIN1111672)

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Biochemical Assay (BCA)

Pureté: >95%

Détail du produit

Séquence: MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA RS VRAQRHTDMP KTQKEVHLKN ASRGSAGNKN YRM

Attributs du produit: Optimized DNA sequence encoding Human Insulin Growth Factor -1mature chain was expressed in Escherichia Coli.

Purification: >95%, as determined by SDS-PAGE and HPLC

niveau d'endotoxine: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Biological Activity Comment: The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤1.0 ng/ml, corresponding to a specific activity of ≥1 x107 units/mg.

Information d'application

Commentaires:

Native Human Insulin Growth Factor-1 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 8 kDa. Recombinant Human IGF-1 is a monomeric protein consisting of 71 amino acid residue subunits. IGF-1 migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: Recombinant Human IGF1was lyophilized from a 0.2 μm filtered PBS solution, pH.4.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Référence

Devi, Dixit: "Clinical Evaluation of Insulin like Growth Factor-I and Vascular Endothelial Growth Factor with Alloplastic Bone Graft Material in the Management of Human Two Wall Intra-Osseous Defects." dans: Journal of clinical and diagnostic research : JCDR, Vol. 10, Issue 9, pp. ZC41-ZC46, (2016) (PubMed).

Détail du antigène

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Autre désignation: Insulin Like Growth Factor-I

Poids moléculaire: 8 kDa

UniProt: P01343

Pathways: Signalisation RTK, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation