CCL2 Protéine

N° du produit ABIN1305100

Détail du produit

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Marque: BD Pharmingen™

Specificité: Upon initial thawing, recombinant mouse MCP-1 (MN 554590) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/mL carrier protein, such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, carrier-protein concentrations of 0.5 - 1 mg/mL are recommended. For use as an ELISA standard, carrier-protein concentrations of 5 - 10 mg/mL are recommended. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible affects in each investigator's experimental system. Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Attributs du produit: Recombinant mouse MCP-1 (MN 554590) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution (pH 7.2) and containing 1.29 mg/mL bovine serum albumin, with no preservatives. Recombinant mouse MCP-1 is > 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Purification: Recombinant mouse MCP-1 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution ( pH 7.2) and containing 1.29 mg/mL bovine serum albumin, with no preservatives. Recombinant mouse MCP-1 is > 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Pureté: ≥ 95 %

Stérilité: 0.22 μm filtered

niveau d'endotoxine: The endotoxin level is ≤ 0.1 ng per µg of mouse MCP-1, as measured in a chromogenic LAL assay.

Information d'application

Indications d'application: ELISA Standard: Recombinant mouse MCP-1 (MN 554590) can be useful as a quantitative standard for measuring mouse MCP-1 protein levels using sandwich ELISA with the purified 2H5 antibody (Cat. No. 551217) as a capture antibody and the biotinylated 4E2/MCP antibody (Cat. No. 554444) as the detection antibody. To obtain linear standard curves, investigators may want to consider using doubling dilutions of recombinant mouse MCP-1 from 4000-30 pg/mL to be included for each ELISA plate. For measuring mouse MCP-1 in serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse MCP-1 Set (Cat. No. 555260).
Bioassay: Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. An activity range encompassing an ED50 = 50 - 800 ng/mL has previously been reported using THP-1 as indicator cells utilizing a calcium flux assay.

Commentaires:

BD Pharmingen™ Recombinant Mouse MCP-1 - Reactivity Ms

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA.

Agent conservateur: Azide free

Conseil sur la manipulation: Mouse monocyte chemoattractant protein-1 (MCP-1), previously termed JE, is a member of the CC chemokine family and a homolog of human MCP-1. MCP-1 is expressed by a variety of cell types including monocyte/macrophages, endothelial cells and mesangial cells. MCP-1 has chemoattractant activity on monocytes, lymphocytes and basophils but is not active on neutrophils.

Stock: -80 °C

Stockage commentaire: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Référence

Rollins: "Chemokines." dans: Blood, Vol. 90, Issue 3, pp. 909-28, (1997) (PubMed).

Ernst, Zhang, Hancock, Rutledge, Corless, Rollins: "Biochemical and biologic characterization of murine monocyte chemoattractant protein-1. Identification of two functional domains." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3541-9, (1994) (PubMed).

Luo, Laning, Hayashi, Hancock, Rollins, Dorf: "Serologic analysis of the mouse beta chemokine JE/monocyte chemoattractant protein-1." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3708-16, (1994) (PubMed).

Rollins, Morrison, Stiles: "Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 11, pp. 3738-42, (1988) (PubMed).

Cochran, Reffel, Stiles: "Molecular cloning of gene sequences regulated by platelet-derived growth factor." dans: Cell, Vol. 33, Issue 3, pp. 939-47, (1983) (PubMed).

Détail du antigène

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Autre désignation: MCP-1 (CCL2 Produits)

Synonymes: GDCF-2 Protein, HC11 Protein, HSMCR30 Protein, MCAF Protein, MCP-1 Protein, MCP1 Protein, SCYA2 Protein, SMC-CF Protein, AI323594 Protein, JE Protein, Scya2 Protein, Sigje Protein, MCP-1A Protein, MCP1A Protein, C-C motif chemokine ligand 2 Protein, chemokine (C-C motif) ligand 2 Protein, C-C motif chemokine 2 Protein, CCL2 Protein, Ccl2 Protein, LOC101120093 Protein

Sujet: Mouse monocyte chemoattractant protein-1 (MCP-1), previously termed JE, is a member of the CC chemokine family and a homolog of human MCP-1. MCP-1 is expressed by a variety of cell types including monocyte/macrophages, endothelial cells and mesangial cells. MCP-1 has chemoattractant activity on monocytes, lymphocytes and basophils but is not active on neutrophils.

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2