Flagellin Protein (FliC)
Aperçu rapide pour Flagellin Protein (FliC) (ABIN1344457)
Antigène
Voir toutes Flagellin (FliC) ProtéinesType de proteíne
Origine
Source
Pureté
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Fonction
- Flagellin (high purity)
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Specificité
- Binds to human and mouse TLR5 (Toll-like receptor 5).
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Réactivité croisée
- Humain, Souris
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niveau d'endotoxine
- <0.001EU/μg purified protein (LAL test, Lonza).
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Biological Activity Comment
- Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1μg /mouse.
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Flagellin (FliC) (AA 2-498) protein (His tag)
FliC Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Flagellin (FliC) proteinFliC Origine: Borrelia burgdorferi Hôte: Escherichia coli (E. coli) Recombinant ELISA, WB, SDS, CP
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- 0.1 mg/mL after reconstitution
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Concentration
- 0.1 mg/mL
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Buffer
- Contains PBS.
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Conseil sur la manipulation
- After reconstitution, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.
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Date de péremption
- 6 months
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- Flagellin (FliC)
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Autre désignation
- Flagellin
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Sujet
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FliC
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
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Poids moléculaire
- ~54kDa (SDS-PAGE)
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UniProt
- P06179
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Pathways
- Inflammasome
Antigène
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