Flagellin Protein (FliC) (AA 1-495) (His tag)

N° du produit ABIN2745676

Détail du produit

Antigène: Flagellin (FliC)

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-495

Origine: Humain, Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette Flagellin protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Specificité: Detected by human and mouse TLR5 (Toll-like receptor 5) and mouse NLRC4 (Nod-like receptor [NLR] family caspase activation and recruitment domain [CARD] domain-containing protein 4).

Réactivité croisée: Humain, Souris

Attributs du produit: The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) is fused at the N-terminus to a His-tag.

Pureté: >95 % (SDS-PAGE)

niveau d'endotoxine: <0.01EU/μg purified protein (LAL test, Lonza).

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (rec.) at concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1μg/mouse. Inflammasome NLRC4 can be activated at a concentration range of 1-5μg/ml on bone-marrow macrophages in the presence of streptolysin O (SLO, 25μg/ml), a protein that allows delivery of exogenous molecules into the cytosol of living cells.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: Lot specific

Buffer: Lyophilized. Contains PBS.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Flagellin (FliC)

Autre désignation: Flagellin (FliC Produits)

Synonymes: hag Protein, flaG Protein, flagellin Protein, flagellin (flic) Protein, flagellin E Protein, flagellin protein flic Protein, fliC Protein, FliC Protein, fliC+F2048 Protein, CJJ81176_1339 Protein, C8J_1256 Protein

Sujet: Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

UniProt: P06179

Pathways: Inflammasome