MANF Protéine

N° du produit ABIN1405920

Cette protéine Recombinant MANF est exprimée dans CHO Cells.

Aperçu rapide pour MANF Protéine (ABIN1405920)

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: CHO Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), Cell Culture (CC), In vivo Studies (in vivo)

Détail du produit

Attributs du produit: Bio-activity testing: Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat &-OHDA model of Parkinson's disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke

Purification: Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Purified Protein is characterized as homogenous material by mass-spectroscopy.

niveau d'endotoxine: Less than 1EU/µg of protein as determined by LAL method

Information d'application

Commentaires:

Synonyms: Mesencephalic astrocyte-derived neurotrophic factor - MANF, ARMET, ARP

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard

Buffer: PBS pH 7.4

Conseil sur la manipulation: Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Détail du antigène

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Autre désignation: MANF

Sujet: MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Poids moléculaire: Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

UniProt: P55145