CD80 Protein (CD80) (C-Term, Extracellular Domain)

N° du produit ABIN1684684

Détail du produit

Antigène: CD80

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: C-Term, Extracellular Domain

Origine: Souris

Source: HEK-293T Cells

Specificité: Optimized DNA sequence encoding extracellular domain of mouse CD80 including a C-terminal 6His tag was expressed in HEK293 cells.

Attributs du produit: Recombinant mouse CD80 is a monomer protein consisting of20 amino acid residue subunits, due to glycosylation migrates as an approximately 45 kDa protein on SDS-PAGE.

Pureté: > 90 %, as determined by SDS-PAGE and HPLC

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Information d'application

Commentaires:

The activity was tested by immobilized recombinant CD80 (1 µg/mL) ability to bind recombinant mouse CD28 in functional ELISA.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 50 mM Tris 100 mM NaCl pH 8.0

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.

Date de péremption: 12-24 months

Détail du antigène

Antigène: CD80

Autre désignation: CD80 (CD80 Produits)

Synonymes: B7 Protein, B7-1 Protein, B7.1 Protein, BB1 Protein, CD28LG Protein, CD28LG1 Protein, LAB7 Protein, B71 Protein, Cd28l Protein, Ly-53 Protein, Ly53 Protein, MIC17 Protein, TSA1 Protein, CD80 molecule Protein, Cd80 molecule Protein, CD80 antigen Protein, CD80 Protein, Cd80 Protein

Sujet: SCF is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and earlydifferentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co-stimulates with other cytokines. The receptor for SCF, designated SCFR (CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL7, Epo and some other growth and differentiation factors. In combination with IL7 SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells (see also: Chemotaxis), for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.

UniProt: Q00609

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints