FGF8 Protein (AA 23-215)

N° du produit ABIN2017830

Protéine Recombinant FGF8 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour FGF8 Protein (AA 23-215) (ABIN2017830)

Antigène: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % by SDS-PAGE and HPLC analyses.

Détail du produit

Attributs du protein: AA 23-215

Attributs du produit: ED50 < 5.0 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 1 μg/mL of heparin, corresponding to a specific activity of > 2.0x 10^5 units/mg.
AA 23-215, expressed with an N-terminal Met.

niveau d'endotoxine: < 0.2 EU/μg, determined by LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Stock: -80 °C

Stockage commentaire: Lyophilized recombinant human Fibroblast Growth Factor-8 (rhFGF-8) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhFGF-8 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Autre désignation: Fibroblast Growth Factor-8 (FGF-8)

Sujet: Fibroblast Growth Factor-8 (FGF-8) is a heparin-binding growth factor of the FGF family. There are 4 known forms of FGF8 produced by alternative splicing: FGF8a, FGF-8b, FGF-8e and FGF-8f. The human and mouse FGF8b are identical of aa sequences. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF-8 is required for normal brain, eye, ear and limb development during embryogenesis. It is also required for normal development of the gonadotropin- releasing hormone (GnRH) neuronal system.Recombinant human Fibroblast Growth Factor-8 (rhFGF-8) produced in E. coli is a single non-glycosylated polypeptide chain containing 194 amino acids. A fully biologically active molecule, rhFGF-8 has a molecular mass of 22.5 kDa analyzed by reducing SDS-PAGE.
Synonyms: FGF-8b, AIGF, HBGF

Poids moléculaire: 22.5kDa, observed by reducing SDS-PAGE.

UniProt: P55075

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis