Interferon gamma Protein (IFNG) (AA 23-155)

N° du produit ABIN2018007

Cette protéine Recombinant Interferon gamma est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Interferon gamma Protein (IFNG) (AA 23-155) (ABIN2018007)

Antigène: Interferon gamma (IFNG)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Pureté: > 95 % as analyzed by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 23-155

Attributs du produit: ED50<0.15 ng/mL, measured by cytotoxicity assay using WEHI-279 cells.
AA 23-155, expressed with an N-terminal Met.

niveau d'endotoxine: < 1 EU/μg, determined by LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Stock: -80 °C

Stockage commentaire: Lyophilized recombinant mouse IFN gamma (rmIFN-γ) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIFN-γ should be stable up to 1week at 4°C or up to 2 months at -20°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Interferon gamma (IFNG)

Autre désignation: Interferon-gamma (IFN-Gamma)

Sujet: Sharing 41 % sequence identity with human Interferon gamma (hIFN--γ), mouse IFN gamma (mIFN--γ) is a macrophage-activating factor.The active form of IFN--γ is an antiparallel dimer that sets off IFN--γ/JAK/STAT pathway. IFN--γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--γ-induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--γ is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers. Recombinant mouse IFN gamma (rmIFN-γ) produced in E. coli is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--γ has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques.
Synonyms: Type II interferon, T cell interferon, MAF, IFNG, IFG, IFI

Poids moléculaire: 15 kDa, observed by reducing SDS-PAGE.

UniProt: P01580

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy