Epiregulin Protein (EREG) (AA 63-108)

N° du produit ABIN2666804

Cette protéine Recombinant Epiregulin est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Epiregulin Protein (EREG) (AA 63-108) (ABIN2666804)

Antigène: Epiregulin (EREG)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Pureté: > 95 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 63-108

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: ED50 = 0.5 - 3.0 ng/ml, corresponding to a specific activity of 0.33 - 2.0 x 106 units/mg, as determined by induction of BALB/3T3 cell proliferation.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS, pH 7.2.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: Epiregulin (EREG)

Autre désignation: Epiregulin

Sujet: Epiregulin was initially identified as a growth-inhibitory factor from the conditioned medium of the murine tumor cell line NIH3T3/clone T7. It belongs to the EGF family of proteins that includes EGF, TGF-α, heparin-binding EGF like-growth factor (HB-EGF), epigen, epiregulin, betacellulin, neuroregulin, and tomoregulin. It posesses 24-50 % amino acid sequence identity with sequences of other EGF-related growth factors. All the EGF family members are synthesized as type I membrane protein precursors, which can undergo proteolytic cleavage at the plasma membrane to release a mature soluble ectodomain. Epiregulin acts as an autocrine growth factor in human epidermal keratinocytes and it can be induced by HB-EGF, amphiregulin, and TGF-α. Epiregulin is expressed by, and plays a key role in, immune-related responses of keratinocytes and tissue resident macrophages. It has been shown that epiregulin-deficient (EP-/-) mice develop chronic dermatitis. Also, epiregulin is involved in proinflammatory cytokine production in bone marrow-derived macrophages. In addition, epiregulin induces proliferation of human corneal epithelial cells and its expression can be induced in these cells by TGF-α, HB-EGF, AR, and EGF. Epiregulin plays a role in the development of hyperkeratosis during the pathogenesis of middle ear cholesteatoma, and it is overexpressed in psoriatic epidermis. Epiregulin polymorphisms seem to be associated with susceptibility to different clinical phenotypes of TB, and epiregulin modulates the innate immune responses of TB.

Poids moléculaire: The 47 amino acid recombinant protein has a predicted molecular mass of approximately 5.4 kDa. The DTT-reduced and non-reduced protein migrate at approximately 6 kDa and 8 kDa, respectively, by SDS-PAGE. The predicted N-terminal amino acid is Met.

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation