EPO Protein (AA 28-192)

N° du produit ABIN2666933

Cette protéine Recombinant EPO est exprimée dans CHO Cells.

Aperçu rapide pour EPO Protein (AA 28-192) (ABIN2666933)

Antigène: EPO (Erythropoietin (EPO))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: CHO Cells

Application: ELISA, Flow Cytometry (FACS)

Pureté: > 98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 28-192

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: ED50 = 0.1 - 0.6 ng/ml, corresponding to a specific activity of 1.67 - 10 x 106 units/mg, as determined by the dose dependent stimulation of TF-1 cells proliferation.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: EPO (Erythropoietin (EPO))

Autre désignation: Erythropoietin

Classe de substances: Hormone

Sujet: Human Erythropoietin (EPO) was initially purified from urine of a patient with aplastic anemia. EPO is a glycoprotein composed of 40-60 % carbohydrates, and its molecular weight varies from 30-34 kD depending on the carbohydrate percentage. Hypoxia induces erythropoiesis, and hypoxia inducible factor (HIF) is directly involved in EPO expression. HIF is a heterodimer (HIF-1α, HIF-2α, and β subunits) transcription factor, and it is regulated by HIF-prolyl hydroxylase (HIF-PH) that hydroxylates the α subunits, targeting them for ubiquitination and subsequent degradation. HIF-PH is a sensor of levels of iron, oxygen, and metabolic activity. High levels of HIF protein induce EPO production in the kidney and liver, and mobilization of iron to support erythropoiesis. EPO binds to the EPOR, and transcripts of this receptor have been detected in non-erythroid precursors such as endothelial cells and renal proximal epithelial cells, nevertheless, it has been published that functional erythropoietin receptor is not detected in endothelial, cardiac, neuronal, and renal cells. Several transcriptions factors play a role in the expression of EPOR such as GATA 1, Friend of GATA (Fog1), and the erythroid specific factor SCL/Tal 1. EPO has been used in the treatment of anemia associated with chronic kidney disease, in cancer patients on chemotherapy, and in antiviral HIV therapy.

Poids moléculaire: The 165 amino acid recombinant protein has a predicted molecular mass of approximately 18 kDa. The DTT-reduced and non-reduced protein migrate at approximately 37-57 kDa and 35-55 kDa by SDS-PAGE respectively. The N-terminal amino acid is Ala.

Pathways: Signalistation JAK/STAT, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity