EPO Protein (AVI tag,His tag)

N° du produit ABIN7536398

Détail du produit

Antigène: EPO (Erythropoietin (EPO))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Purification/Conjugué: Cette EPO protéine est marqué à la AVI tag,His tag.

Fonction: Recombinant Mouse Erythropoietin/EPO Protein

Séquence: APPRLICDSR VLERYILEAK EAENVTMGCA EGPRLSENIT VPDTKVNFYA WKRMEVEEQA IEVWQGLSLL SEAILQAQAL LANSSQPPET LQLHIDKAIS GLRSLTSLLR VLGAQKELMS PPDTTPPAPL RTLTVDTFCK LFRVYANFLR GKLKLYTGEV CRRGDR

Specificité: Ala27-Arg192

Stérilité: 0.22 μm filtered

niveau d'endotoxine: <0.001EU/μg

Biological Activity Comment: Recombinant Mouse EPO stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 for this effect is 4.3-17.3 ng/mL.corresponding to a specific activity of 5.78x104-2.33x105units/mg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Concentration: 0.92 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of 20 mM NaAc, pH 4.5

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Détail du antigène

Antigène: EPO (Erythropoietin (EPO))

Autre désignation: Erythropoietin/EPO (EPO Produits)

Synonymes: EPO Protein, EP Protein, MVCD2 Protein, erythropoietin Protein, erythropoietin S homeolog Protein, erythropoietin a Protein, EPO Protein, epo Protein, epo.S Protein, Epo Protein, epoa Protein

Classe de substances: Hormone

Sujet:

Description: Erythropoietin is a member of the EPO / TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. Erythropoietin can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Genetic variation in erythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. It has a longer circulating half-life in vivo. Erythropoietin is being much misused as a performance-enhancing drug in endurance athletes.

Name: Erythropoietin,EPO

ID gène: 13856

UniProt: P07321

Pathways: Signalistation JAK/STAT, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity