Caspase 3 Protein (CASP3)

N° du produit ABIN2688833

Protéine Recombinant Caspase 3 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Caspase 3 Protein (CASP3) (ABIN2688833)

Antigène: Caspase 3 (CASP3)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Fluorescence Spectroscopy (FS)

Pureté: ≥ 95%

Détail du produit

Marque: BD Pharmingen™

Purification: Active caspase-3 was expressed in E.coli and purified. When expressed in E. coli, caspase-3 will spontaneously undergo autoprocessing to yield the subunits characteristic of the active enzyme. Cleavage of the Ac-DEVD-AMC substrate by recombinant human caspases-3, -6, -7, and -8. The activity of the caspases was analyzed by spectrofluorometry using an excitation at 380 nm and an emission wwavelength of 430-460 nm (peak is at 440 nm).

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

BD Pharmingen™ Recombinant Human Active Caspase-3

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 50 mM Tris ( pH 8.0) with 100 mM NaCl and 50 mM imidazole

Stock: 4 °C,-80 °C

Stockage commentaire: Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. The thawed active enzyme is generally stable at 4°C for about one week.

Détail du antigène

Antigène: Caspase 3 (CASP3)

Autre désignation: Caspase-3

Pathways: Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation