Cyclin D1 Protein (CCND1) (DYKDDDDK Tag)

N° du produit ABIN2712724

Cette protéine Recombinant Cyclin D1 est exprimée dans Insect cells (Sf9).

Aperçu rapide pour Cyclin D1 Protein (CCND1) (DYKDDDDK Tag) (ABIN2712724)

Antigène: Cyclin D1 (CCND1)

Type de proteíne: Recombinant

Origine: Humain

Source: Insect cells (Sf9)

Application: Antibody Production (AbP), Standard (STD)

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Détail du produit

Purification/Conjugué: Cette Cyclin D1 protéine est marqué à la DYKDDDDK Tag.

Attributs du produit:

• Recombinant human Cyclin D1 (full length, C-term flag tag) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 7 months from receipt of products under proper storage and handling conditions.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: Cyclin D1 (CCND1)

Autre désignation: Cyclin d1

Sujet: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

Poids moléculaire: 34 kDa

NCBI Accession: NP_444284

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling