Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human Insulin-like growth factor I / IGF1 (transcript variant 1) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
> 95 % , as determined by Coomassie stained SDS-PAGE
niveau d'endotoxine
Less than 0.01 ng per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 3-15 ng/ml, corresponding to a specific activity of 0.6-3.3 x 105 units/mg, determined by the dose dependent stimulation of MCF-7 cell proliferation.
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Concentration
> 50 μg/mL
Buffer
1 x PBS
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.