IL12B Protéine

N° du produit ABIN2723546

Protéine Recombinant IL12B exprimée dans Insect cells (Sf9).

Aperçu rapide pour IL12B Protéine (ABIN2723546)

Antigène: IL12B (Interleukin 12 beta (IL12B))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Insect cells (Sf9)

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Pureté: 95 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human Interleukin 12b (Il12b) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

niveau d'endotoxine: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 0.10 to 0.20 ng/ml, corresponding to a specific activity of 1.0 - 0.5 x 10^7 units/mg, determined by the dose dependent stimulation of IFN&gamma production by mouse splenocytes.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Concentration: > 50 μg/mL

Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: IL12B (Interleukin 12 beta (IL12B))

Autre désignation: Interleukin 12b (Il12b)

Poids moléculaire: 57.5 kDa

NCBI Accession: NP_032378

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation