IL-4 Protein (Transcript Variant 1)

N° du produit ABIN2723722

Cette protéine Recombinant IL-4 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-4 Protein (Transcript Variant 1) (ABIN2723722)

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)

Pureté: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Détail du produit

Attributs du protein: Transcript Variant 1

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human Interleukin-4 / IL4 (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

niveau d'endotoxine: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 as determined by the dose-dependent stimulation of human TF-1 cells is less than or equal to 0.2 ng/ml, corresponding to a specific activity of > 5 x 10^6 units/mg.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Conseil sur la manipulation: Resuspend the protein in the desired concentration in proper buffer

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Autre désignation: Interleukin-4,il4

Sujet: The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Poids moléculaire: 15.1 kDa

NCBI Accession: NP_000580

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation