VEGFA Protein (Transcript Variant 2)

N° du produit ABIN2735216

Protéine Recombinant VEGFA exprimée dans HEK-293 Cells.

Aperçu rapide pour VEGFA Protein (Transcript Variant 2) (ABIN2735216)

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Application: Antibody Production (AbP), Functional Studies (Func), Standard (STD), Protein Interaction (PI)

Pureté: > 98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: Transcript Variant 2

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human VEGF-A (transcript variant 2) protein expressed in HEK293.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

niveau d'endotoxine: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 1- 4 ng/ml, corresponding to a specific activity of 0.25 - 1 x 10^6 units/mg, determined by the dose dependent stimulation of HUVEC cells proliferation.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Concentration: > 50 μg/mL

Buffer: 5 mM citric acid, 5 mM NaHPO4, 0.15 M NaCl, pH 4.0

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Autre désignation: Vegf-A

Poids moléculaire: 19.3 kDa

NCBI Accession: NP_033531

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling