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SNCA Protein (full length)

Name: SNCA Protein (full length)
Brand: antibodies-online
SKU: ABIN5065829
Price: 712.5 EUR
Availability: InStock

Cette protéine Recombinant SNCA est exprimée dans Escherichia coli (E. coli).

SDS-PAGE of ~14 kDa Active Human Recombinant Alpha Synuclein Protein Aggregate . (SNCA Protein (full length))

SDS

Toxicity results comparing Active Human Recombinant Alpha Synuclein Pre-formed Fibrils (Type 2) (Catalog No. (SNCA Protein (full length))

Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 µg/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 µg/ml (A-C). (SNCA Protein (full length))

IF/ICC

Primary rat hippocampal neurons show lewy body inclusion formation when treated with Type 1 Alpha Synuclein Protein Pre-formed Fibrils (ABIN5065829, ABIN5065830 and ABIN5564165) at 4 μg/mL (D-F), but not when treated with Type 2 Alpha Synuclein Protein Pre-formed Fibrils (ABIN5065829, ABIN5065830 and ABIN5564165) at 4 μg/mL (A-C). (SNCA Protein (full length))

ATTO633 fluorescently-labelled alpha synuclein PFFs (ABIN5065829, ABIN5065830 and ABIN5564165) were taken up, transported into the soma, and induced alpha synuclein aggregation in mouse neurocortical primary cells. (SNCA Protein (full length))

Confocal imaging shows NeuN+ (mature) primary cortical neurons filled with ATTO633 fluorescently-labelled alpha synuclein PFFs (ABIN5065829, ABIN5065830 and ABIN5564165). (SNCA Protein (full length))

Immunohistochemistry analysis of rat brain injected with Type 1 human alpha synuclein PFFs (ABIN5065829, ABIN5065830 and ABIN5564165). (SNCA Protein (full length))

IHC

TEM of Type 1 Alpha Synuclein Pre-formed Fibrils (PFFs) (ABIN5065829, ABIN5065830 and ABIN5564165) (SNCA Protein (full length))

Type 1 alpha synuclein Pre-formed Fibrils (ABIN5065829, ABIN5065830 and ABIN5564165) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (ABIN5065829, ABIN5065830 and ABIN5564165). (SNCA Protein (full length))

10 images

N° du produit ABIN5065829

Aperçu rapide pour SNCA Protein (full length) (ABIN5065829)

Antigène

Voir toutes SNCA Protéines

SNCA (Synuclein, alpha (SNCA))

Type de proteíne

Recombinant

Activité biologique

Active

Origine

Humain

Source

Escherichia coli (E. coli)

Application

SDS-PAGE (SDS), Western Blotting (WB), In vitro Assay (in vitro), In vivo Studies (in vivo)

Pureté

>95% pure using SDS-PAGE analysis.

Discover our top product SNCA Protéine

Attributs du protein

full length

Séquence

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Specificité

~14.46 kDa

Purification

Ion-exchange Purified

Biological Activity Comment

Endogenous alpha-synuclein phosphorylation. 100 μM alpha synuclein protein monomer (SPR-321) seeded with 10 nM alpha synuclein protein PFF (SPR-322) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
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Produit

Système d'expression

Conjugué

Origin

Prix à partir de

SNCA Protein (AA 1-140) (His tag)

Système d'expression HEK-293 Cells

Conjugué His tag

Origin Human

Prix à partir de 13.711,36 €

SNCA Protein (AA 1-140) (Strep Tag)

Système d'expression Cell-free protein synthesis (CFPS)

Conjugué Strep Tag

Origin Human

Prix à partir de 18.117,43 €

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Synuclein, alpha (SNCA) (A53T) (Active) protein
SNCA Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant SDS, WB, in vitro, in vivo Active

Synuclein, alpha (SNCA) (full length) (Active) protein
SNCA Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant SDS, WB, in vitro, in vivo Active

Synuclein, alpha (SNCA) (AA 1-140) protein (His tag)
custom-made SNCA Origine: Humain Hôte: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Synuclein, alpha (SNCA) (AA 1-140) protein (His tag)
custom-made SNCA Origine: Souris Hôte: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Synuclein, alpha (SNCA) protein
SNCA Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE

Synuclein, alpha (SNCA) (AA 1-140) protein (His tag)
SNCA Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA

Synuclein, alpha (SNCA) (AA 1-140) protein (His-IF2DI Tag)
SNCA Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA

Synuclein, alpha (SNCA) (active) protein
SNCA Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 95 % pure SDS

Synuclein, alpha (SNCA) (AA 31-140) protein (GST tag)
SNCA Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA

Synuclein, alpha (SNCA) (AA 1-140) protein (GST tag)
SNCA Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA

Indications d'application

Optimal working dilution should be determined by the investigator.

Commentaires

Certified >95% pure using SDS-PAGE analysis.

Restrictions

For Research Use only
Concentration

Lot specific

Buffer

PBS

Stock

-80 °C
Antigène

SNCA (Synuclein, alpha (SNCA))

Autre désignation

Alpha Synuclein

Sujet

Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions, inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).

Poids moléculaire

14.46 kDa

ID gène

6622

NCBI Accession

NP_000336

UniProt

P37840

Pathways

Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity