GFPT1 Protein (AA 332-699) (His tag)
Aperçu rapide pour GFPT1 Protein (AA 332-699) (His tag) (ABIN5853831)
Antigène
Voir toutes GFPT1 ProtéinesType de proteíne
Origine
Source
Application
Pureté
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Attributs du protein
- AA 332-699
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Purification/Conjugué
- Cette GFPT1 protéine est marqué à la His tag.
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Séquence
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QQIMKGN FSSFMQKEIF EQPESVVNTM RGRVNFDDYT VNLGGLKDHI KEIQRCRRLI LIACGTSYHA GVATRQVLEE LTELPVMVEL ASDFLDRNTP VFRDDVCFFL SQSGETADTL MGLRYCKERG ALTVGITNTV GSSISRETDC GVHINAGPEI GVASTKAYTS QFVSLVMFAL MMCDDRISMQ ERRKEIMLGL KRLPDLIKEV LSMDDEIQKL ATELYHQKSV LIMGRGYHYA TCLEGALKIK EITYMHSEGI LAGELKHGPL ALVDKLMPVI MIIMRDHTYA KCQNALQQVV ARQGRPVVIC DKEDTETIKN TKRTIKVPHS VDCLQGILSV IPLQLLAFHL AVLRGYDVDF PRNLAKSVTV E -
Purification
- > 95% by SDS-PAGE
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 50 % glycerol, 2 mM DTT, 2 mM EDTA
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))
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Autre désignation
- GFPT1
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Sujet
- GFPT1 is the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. Recombinant human GFPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Poids moléculaire
- 43.7 kDa (391aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001231639
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Pathways
- ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process
Antigène
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