Interferon gamma Protein (IFNG) (AA 24-161)
Aperçu rapide pour Interferon gamma Protein (IFNG) (AA 24-161) (ABIN7529268)
Antigène
Voir toutes Interferon gamma (IFNG) ProtéinesType de proteíne
Activité biologique
Origine
Source
Application
Pureté
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Attributs du protein
- AA 24-161
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Séquence
- MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG
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niveau d'endotoxine
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured in a cytotoxicity assay using WiDr cells. The ED50 range ≤ 0.5ng/ml.
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Interferon gamma (IFNG) (AA 24-161) protein (His-Avi Tag)
IFNG Origine: Humain Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interferon gamma (IFNG) (AA 24-161) protein (His-Avi Tag,Biotin)IFNG Origine: Humain Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interferon gamma (IFNG) (Active) proteinIFNG Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interferon gamma (IFNG) (AA 23-155) (Active) proteinIFNG Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by reducing SDS-PAGE. Active
Interferon gamma (IFNG) proteinIFNG Origine: Souris Hôte: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interferon gamma (IFNG) (AA 1-144) protein (His tag)IFNG Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interferon gamma (IFNG) (AA 24-174) protein (His tag)IFNG Origine: Hamster Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interferon gamma (IFNG) (AA 24-166) protein (His tag)IFNG Origine: Porc Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interferon gamma (IFNG) (AA 23-155) (Active) protein (His tag)IFNG Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE. WB, ELISA Active
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Commentaires
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In PBS buffer ( pH 7.4) containing 10 % glycerol, 1 mM DTT
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Interferon gamma (IFNG)
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Autre désignation
- IFN-gamma/IFNG
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Sujet
- IFNG is a member of the type II interferon family. This protein is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia. Recombinant human IFNG protein was expressed in E. coli and purified by using conventional chromatography techniques.
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Poids moléculaire
- 16.3 kDa (139aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_000610
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UniProt
- P01579
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Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
Antigène
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