EPO Protein (His tag)

N° du produit ABIN6122629

Protéine Recombinant EPO exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour EPO Protein (His tag) (ABIN6122629)

Antigène: EPO (Erythropoietin (EPO))

Type de proteíne: Recombinant

Origine: Singe

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB)

Pureté: > 95 %

Détail du produit

Purification/Conjugué: Cette EPO protéine est marqué à la His tag.

Fonction: Monkey Erythropoietin (EPO) Protein

Séquence: APP RLICDSRVLE RYLLEAKEAE NVTMGCSESC SLNENITVPD TKVNFYAWKR MEVGQQAVEV WQGLALLSEA VLRGQAVLAN SSQPFEPLQL HMDKAISGLR SITTLLRALG AQQEAISLPD AASAAPLRTI TADTFCKLFR VYSNFLRGKL KLYTGEACRR GDR

niveau d'endotoxine: < 1.0 EU per 1 μg (determined by the LAL method)

Biological Activity Comment: Not tested

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01 % Sarcosyl, 5 % Trehalose.

Stock: 4 °C,-80 °C

Stockage commentaire: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: EPO (Erythropoietin (EPO))

Autre désignation: Erythropoietin

Classe de substances: Hormone

Poids moléculaire: Calculated MW: 22.1 kDa
Observed MW (SDS-PAGE): 27 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.

ID gène

719294

UniProt: Q28513

Pathways: Signalistation JAK/STAT, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity