Flagellin Protein (FliC) (Mutant) (His tag)

N° du produit ABIN6253486

Cette protéine Recombinant Flagellin est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Flagellin Protein (FliC) (Mutant) (His tag) (ABIN6253486)

Antigène: Flagellin (FliC)

Type de proteíne: Recombinant

Origine: Humain, Souris

Source: Escherichia coli (E. coli)

Pureté: >95 % (SDS-PAGE)

Détail du produit

Attributs du protein: Mutant

Purification/Conjugué: Cette Flagellin protéine est marqué à la His tag.

Fonction: Flagellin (TLR5 Mutant) (rec.)

Specificité: The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.

Réactivité croisée: Humain, Souris

Attributs du produit: The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.

niveau d'endotoxine: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Does not activate TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Conseil sur la manipulation: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

Stock: 4 °C,-20 °C

Stockage commentaire:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Flagellin (FliC)

Autre désignation: Flagellin

Sujet:

FliC (TLR5 Mutant)

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) or the intracellular NLRC4 protein. The Flagellin (TLR5 Mutant) (rec.) cannot be detected by human and mouse TLR5. It has not been tested on NLRC4.

Pathways: Inflammasome