PCNA Protein (AA 1-261)

N° du produit ABIN667068

Cette protéine Recombinant PCNA est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour PCNA Protein (AA 1-261) (ABIN667068)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 1-261

Séquence: MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S

Attributs du produit: PCNA, 1-261 aa, Human, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 7.5) containing 2 mM EDTA, 20 % glycerol

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by uV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E. coli and purified by conventional chromatography techniques.

Poids moléculaire: 28.7 kDa (261aa) confirmed by MALDI-TOF

NCBI Accession: NP_002583

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy