DDIT4 Protein (AA 1-232) (His tag)

N° du produit ABIN667871

Cette protéine Recombinant DDIT4 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour DDIT4 Protein (AA 1-232) (His tag) (ABIN667871)

Antigène: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 90 %

Détail du produit

Attributs du protein: AA 1-232

Purification/Conjugué: Cette DDIT4 protéine est marqué à la His tag.

Séquence: MGSSHHHHHH SSGLVPRGSH MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC

Attributs du produit: DDIT4, 1-232aa, Human, His tag, E.coli

Purification: > 90% by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 5 mM DTT, 1 mM EDTA, 30 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Autre désignation: DDIT4

Sujet: DDIT4, also known as Dig2 or REDD1, is thought to have function in the regulation of reactive oxygen species. In response to stress due to DNA damage and glucocorticoid treatment, DDIT4 is upregulated at the transcriptional level. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. Recombinant human DDIT4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 27.5 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NCBI Accession: NP_061931

Pathways: Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process