c-FOS Protein (GST tag)

N° du produit ABIN6699563

N° du produit (Fournisseur): 009-001-r95s

Cette protéine Recombinant c-FOS est exprimée dans Insect cells (Sf9).

Aperçu rapide pour c-FOS Protein (GST tag) (ABIN6699563)

Antigène: c-FOS (c-Fos) (c-Fos)

Type de proteíne: Recombinant

Origine: Humain

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Pureté: >90%

Détail du produit

Purification/Conjugué: Cette c-FOS protéine est marqué à la GST tag.

N° du produit (Fournisseur): 009-001-r95s

Fournisseur: Rockland

Fonction: FOS recombinant protein-GST fusion protein

Purification: Recombinant full-length human FOS was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Information d'application

Indications d'application:

Application Note: FOS Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. FOS Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~78 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: Kinase Assay-User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: FOS Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: c-FOS (c-Fos) (c-Fos)

Autre désignation: FOS

Sujet:

Synonyms: AP-1, c-FOS, Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7

Background: FOS is a member of the FOS gene family that consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. FOS gene encodes a leucine zipper protein that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation (1). In some cases, expression of the FOS gene has also been associated with apoptotic cell death. FOS overexpression leads to decreased phosphorylation and dimerization of STAT1, which in turn downregulates p21 gene expression (2). This regulatory pathway may enhance the proliferation of lymphocytes in rheumatoid arthritis patients. FOS Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, and Neurobiology research.

NCBI Accession: NM_005252

Pathways: S100 Proteins