CD40 Ligand Protein (CD40LG)

N° du produit ABIN6699578

N° du produit (Fournisseur): 009-001-u68-0010

Cette protéine Recombinant CD40 Ligand est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CD40 Ligand Protein (CD40LG) (ABIN6699578)

Antigène: CD40 Ligand (CD40LG)

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 98,00%

Détail du produit

N° du produit (Fournisseur): 009-001-u68-0010

Fournisseur: Rockland

Fonction: Human CD40 Ligand Recombinant Protein

Purification: CD40 Ligand purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is tested by HEK-blue ™ CD40L Activity. Acceptable Level <50ng/mL, >2.0x10^4 units/mg. Additionally testing by ELISA: IL-8 production by human PBMCs.

Information d'application

Indications d'application:

Application Note: CD40 Ligand Recombinant Protein has been tested by SDS-PAGE and biological assay and is suitable as a control for polyclonal or monoclonal anti-CD40 Ligand in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD40LG

Sujet:

Synonyms: TNFSF5, TRAP, CD154, gp39, T-BAM

Background: CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-α, and IL-2. Recombinant human CD40 Ligand is a non-glycosylated protein, containing 149 amino acids, with a molecular weight of 16.3 kDa.

UniProt: P29965

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints