Cyclin D1 Protein (CCND1) (GST tag)

N° du produit ABIN6699739

N° du produit (Fournisseur): 009-001-153s

Cette protéine Recombinant Cyclin D1 est produite dans Escherichia coli (E. coli). Il y a 1 publication disponible.

Aperçu rapide pour Cyclin D1 Protein (CCND1) (GST tag) (ABIN6699739)

Antigène: Cyclin D1 (CCND1)

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >85%

Détail du produit

Purification/Conjugué: Cette Cyclin D1 protéine est marqué à la GST tag.

N° du produit (Fournisseur): 009-001-153s

Fournisseur: Rockland

Fonction: CyclinD1 recombinant protein-GST fusion protein

Purification: Recombinant full-length human CyclinD1 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Information d'application

Indications d'application:

Application Note: CyclinD1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CyclinD1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 61 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: CyclinD1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Référence

Del Toro, Fernandez-Ruiz, Mignacca, Kalegari, Rowell, Igelmann, Saint-Germain, Benfdil, Lopes-Paciencia, Brakier-Gingras, Bourdeau, Ferbeyre, Lessard: "Ribosomal protein RPL22/eL22 regulates the cell cycle by acting as an inhibitor of the CDK4-cyclin D complex." dans: Cell cycle (Georgetown, Tex.), Vol. 18, Issue 6-7, pp. 759-770, (2020) (PubMed).

Détail du antigène

Antigène: Cyclin D1 (CCND1)

Autre désignation: CCND1

Sujet:

Synonyms: BCL1, PRAD1, U21B31, D11S287E, G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene

Background: CyclinD1 is a member of the cyclin family of proteins that are important regulators cyclin-dependent protein kinases (CDKs) (1). CyclinD1 forms a complex and functions as a regulatory subunit of CDK4 or CDK6 which are important during cell cycle G1/S transition. CyclinD1 has been shown to interact with the tumor suppressor protein Rb and the expression of CyclinD1 is positively regulated by Rb. Mutations, amplification and overexpression of CyclinD1 have been frequently observed in a variety of tumors and suggesting a role of this protein in tumorigenesis (2). CyclinD1 is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

NCBI Accession: NM_053056

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling