FGF1 Protéine

N° du produit ABIN6699829

N° du produit (Fournisseur): 009-001-u79-0050

Cette protéine Recombinant FGF1 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour FGF1 Protéine (ABIN6699829)

Antigène: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 97,00%

Détail du produit

N° du produit (Fournisseur): 009-001-u79-0050

Fournisseur: Rockland

Fonction: Human Fibroblast Growth Factor acidic Recombinant Protein

Purification: Fibroblast Growth Factor acidic purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically 2.0 ng/mL.

Information d'application

Indications d'application:

Application Note: Fibroblast Growth Factor acidic Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor acidic in immunological assays. Buffer formation: 10 mM sodium phosphate, 150 mM sodium sulfate, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 50μL

Concentration: 0.1 mg/mL

Buffer: Buffer formation: 10 mM sodium phosphate, 150 mM sodium sulfate, pH 7.5.

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Autre désignation: FGF1

Sujet:

Synonyms: Heparin-binding growth factor 1 (HBGF-1), Beta-endothelial growth factor, ECGF-beta, acidic fibroblast growth factor (aFGF)

Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. FGF-acidic, or FGF-1, is a particularly potent inducer of DNA synthesis and has chemotactic activities. Recombinant human FGF acidic is a non-glycosylated protein, containing 141 amino acids, with a molecular weight of 16 kDa.

UniProt: P05230

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway