IGF1 Protéine

N° du produit ABIN6699960

N° du produit (Fournisseur): 009-001-v21-0020

Protéine Recombinant IGF1 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IGF1 Protéine (ABIN6699960)

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 98,00%

Détail du produit

N° du produit (Fournisseur): 009-001-v21-0020

Fournisseur: Rockland

Fonction: Human Insulin-like Growth Factor I Recombinant Protein

Purification: Insulin-like Growth Factor I purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse FDC-P1 cells is typically less than 1.0 ng/mL.

Information d'application

Indications d'application:

Application Note: Insulin-like Growth Factor I Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Insulin-like Growth Factor I in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Autre désignation: IGF1

Sujet:

Synonyms: Somatamedin C, mechano growth factor (MGF), IGF-IA

Background: Insulin-like Growth Factor I, IGF-I, is a growth factor produced by the liver when stimulated with growth hormone and can be found circulating throughout the body . IGF-I activates the IGF-I receptor (IGF1R) and the insulin receptor to mediate growth of almost every cell of the body. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Mature human IGF-I is 100 % homologous with bovine and porcine proteins. Recombinant human IGF-I is a non-glycosylated protein, containing 70 amino acids, with a molecular weight of 7.7 kDa.

UniProt: P05019

Pathways: Signalisation RTK, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation