PDGFA Protéine

N° du produit ABIN6700396

N° du produit (Fournisseur): 010-001-w08-0010

Cette protéine Recombinant PDGFA est produite dans Escherichia coli (E. coli).

Aperçu rapide pour PDGFA Protéine (ABIN6700396)

Antigène: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 97,00%

Détail du produit

N° du produit (Fournisseur): 010-001-w08-0010

Fournisseur: Rockland

Fonction: Mouse Platelet Derived Growth Factor AA Recombinant Protein

Purification: platelet Derived Growth Factor AA purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of Balb/c 3T3 cells and is typically 8-10 ng/mL.

Information d'application

Indications d'application:

Application Note: Platelet Derived Growth Factor-AA Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-AA in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Autre désignation: Pdgfa

Sujet:

Synonyms: PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide

Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant mouse PDGF-AA is a non-glycosylated, disulfide-linked homodimer, containing two 126 amino acid chains, with a total molecular weight of 28.9 kDa.

UniProt: P20033

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling