TRAIL Protéine

N° du produit ABIN6700756

N° du produit (Fournisseur): 009-001-w34-0010

Cette protéine Recombinant TRAIL est produite dans Escherichia coli (E. coli).

Aperçu rapide pour TRAIL Protéine (ABIN6700756)

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 90,00%

Détail du produit

N° du produit (Fournisseur): 009-001-w34-0010

Fournisseur: Rockland

Fonction: Human TRAIL Recombinant Protein

Purification: TRAIL purity was determined to be greater than 90% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to induce apoptotic cell death in TRAIL-sensitive RPMI-8226 cells and is typically 10-15 ng/mL.

Information d'application

Indications d'application:

Application Note: TRAIL Recombinant Protein ha been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-TRAIL in immunological assays. Product is provided as lyophilized powder in 0.05M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer: Product is provided as lyophilized powder in 0.05M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Autre désignation: TNFSF10

Sujet:

Synonyms: Apo2 Ligand (Apo2L), TL2, TNF-related apoptosis-inducing ligand, CD253

Background: TNF-Related Apoptosis-Inducing Ligand (TRAIL) is produced by a wide variety of cell types and is shown to be a cytotoxic protein that induces apoptosis in tumor cells through activation of the death receptors, DR4 and DR5. Human TRAIL is active on mouse cells. Recombinant human TRAIL is a non-glycosylated protein, containing the 168 amino acid extracellular portion of TRAIL that is homologous to TNF. It has a molecular weight of 19.5 kDa.

UniProt: P50591

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity