LIF Protéine

N° du produit ABIN7199480

Cette protéine Recombinant LIF est exprimée dans HEK-293 Cells.

Aperçu rapide pour LIF Protéine (ABIN7199480)

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Type de proteíne: Recombinant

Origine: Souris

Source: HEK-293 Cells

Pureté: >90 % as determined by SDS-PAGE.

Classe de qualité: MALS verified

Détail du produit

Fonction: ActiveMax® Mouse LIF Protein, Tag Free (MALS verified)

Séquence: Ser 24 - Phe 203

Attributs du produit: ActiveMax® Mouse LIF, Tag Free (LIF-M5219) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Phe 203 (Accession # P09056-1).

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: This protein carries no "tag". The protein has a calculated MW of 20.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Stock: -20 °C

Stockage commentaire: -20°C

Détail du antigène

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Autre désignation: LIF

Sujet: Synonyms: LIF,CDF,DIA,HILDA,MLPLI,
Description: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

Poids moléculaire: 20.0 kDa

NCBI Accession: NP_032527

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding