ERN1 Protein (AA 465-977) (His-GST)

N° du produit ABIN7317060

Cette protéine Recombinant ERN1 est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour ERN1 Protein (AA 465-977) (His-GST) (ABIN7317060)

Antigène: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 80 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 465-977

Purification/Conjugué: Cette ERN1 protéine est marqué à la His-GST.

Fonction: Recombinant Human ERN1/IRE1 Protein (aa 465-977, His & GST Tag)

Séquence: Pro 465-Leu 977

Attributs du produit: A DNA sequence encoding the human ERN1 isoform 1 (O75460-1) cytoplasmic domain (Pro 465-Leu 977) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 5 nmol/min/mg using MBP as substrate

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Autre désignation: ERN1/IRE1

Sujet: IRE1,IRE1P,IRE1a,hIRE1p,The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.

Poids moléculaire:

Calculated MW: 86.0 kDa

Observed MW: 90 kDa

ID gène: 2081

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response