EPH Receptor B2 Protein (EPHB2) (AA 570-987) (His-GST)

N° du produit ABIN7317086

Protéine Recombinant EPH Receptor B2 exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour EPH Receptor B2 Protein (EPHB2) (AA 570-987) (His-GST) (ABIN7317086)

Antigène: EPH Receptor B2 (EPHB2)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 570-987

Purification/Conjugué: Cette EPH Receptor B2 protéine est marqué à la His-GST.

Fonction: Recombinant Human EphB2 Protein (aa 570-987, His &GST Tag)

Séquence: Gly570-Val987

Attributs du produit: A DNA sequence encoding the human EPHB2 (P29323-3) (Gly570-Val987) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 120 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 3 mM DTT, 10 % glycerol

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: EPH Receptor B2 (EPHB2)

Autre désignation: EphB2

Sujet: CAPB,DRT,EK5,EPHB2,EPHT3,ERK,Ephrin type-B receptor 2,Hek5,PCBC,Tyro5,Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Poids moléculaire:

Calculated MW: 75.2 kDa

Observed MW: 65 kDa

ID gène: 2048

UniProt: P29323

Pathways: Signalisation RTK, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins