EPO Protein (His tag)

N° du produit ABIN7320904

Protéine Recombinant EPO exprimée dans Human Cells.

Aperçu rapide pour EPO Protein (His tag) (ABIN7320904)

Antigène: EPO (Erythropoietin (EPO))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Human Cells

Pureté: >95 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette EPO protéine est marqué à la His tag.

Fonction: Recombinant Mouse Erythropoietin/EPO (C-6His)

Séquence: Ala27-Arg192

Attributs du produit: Recombinant Mouse Erythropoietin is produced by our Mammalian expression system and the target gene encoding Ala27-Arg192 is expressed with a 6His tag at the C-terminus.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.35 ng/mL.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: EPO (Erythropoietin (EPO))

Autre désignation: Erythropoietin/EPO

Classe de substances: Hormone

Sujet:

Background: Erythropoietin (EPO) is a glycoprotein hormone that is principally known for its role in erythropoiesis, where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells. Erythropoietin is a member of the EPO/TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. The differentiation of CFU-E (Colony Forming Unit-Erythroid) cells into erythrocytes can only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by the liver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation of smooth muscle, neural protection during hypoxia, and stimulation of certain B cells. Genetic variation in erythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy.

Synonym: Erythropoietin, Epoetin, EPO

Poids moléculaire: 19.4 kDa

UniProt: Q0VED9

Pathways: Signalistation JAK/STAT, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity