Interferon gamma Protein (IFNG) (AA 23-155) (His tag)

N° du produit ABIN7320567

Cette protéine Recombinant Interferon gamma est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Interferon gamma Protein (IFNG) (AA 23-155) (His tag) (ABIN7320567)

Antigène: Interferon gamma (IFNG)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Pureté: > 98 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 23-155

Purification/Conjugué: Cette Interferon gamma protéine est marqué à la His tag.

Fonction: Recombinant Mouse Interferon γ/IFNG Protein (E.coli)

Séquence: His23-Cys155

Attributs du produit: Recombinant Mouse Interferon gamma is produced by our E.coli expression system and the target gene encoding His23-Cys155 is expressed.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to anti-viral assay in L-929 cells infected with encephalomyocarditis (EMC) virus.The ED₅₀ for this effect is <0.5 ng/mL.The specific activity of recombinant mouse IFN gamma is approximately >2x 10⁶ IU/mg.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Interferon gamma (IFNG)

Autre désignation: Interferon gamma/IFNG

Sujet: IFN-gamma,Ifng,Interferon gamma,Mouse Ifng is a secreted protein which belongs to the type I I (or gamma) interferon family. IFNG is produced by lymphocytes and activated by specific antigens or mitogens. In addition to having antiviral activity, IFNG also has important immunoregulatory functions. It is a potent activator of macrophages and has antiproliferative effects on transformed cells. It can potentiate the antiviral and antitumor effects of the type I interferons. Genetic variation in IFNG is associated with the risk of aplastic anemia (AA) which is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

Poids moléculaire:

Calculated MW: 16.5 kDa

Observed MW: 16 kDa

ID gène: 15978

UniProt: P01580

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy