NGFB Protein (AA 130-239)

N° du produit ABIN7320689

Cette protéine Recombinant NGFB est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour NGFB Protein (AA 130-239) (ABIN7320689)

Antigène: NGFB (Nerve Growth Factor beta (NGFB))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 130-239

Fonction: Recombinant Mouse β-NGF/NGFB Protein

Séquence: Met130-Arg239

Attributs du produit: Recombinant Mouse beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Met130-Arg239 is expressed. 

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 68.52 ng/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: NGFB (Nerve Growth Factor beta (NGFB))

Autre désignation: beta-NGF/NGFB

Sujet: Beta-NGF,Beta-Nerve Growth Factor,NGF,NGFB,NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90 % homology at the amino acid level with human beta -NGF and 95.8 % with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.

Poids moléculaire:

Calculated MW: 12.4 kDa

Observed MW: 13 kDa

ID gène: 18049

UniProt: P01139

Pathways: Signalisation NF-kappaB, Signalisation RTK, Regulation of Cell Size