Nicastrin Protein (NCSTN) (His tag)

N° du produit ABIN7197196

Cette protéine Recombinant Nicastrin est exprimée dans HEK-293 Cells.

Aperçu rapide pour Nicastrin Protein (NCSTN) (His tag) (ABIN7197196)

Antigène: Nicastrin (NCSTN)

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 93 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette Nicastrin protéine est marqué à la His tag.

Fonction: Recombinant Human Nicastrin/NCSTN Protein (His Tag)

Séquence: Met 1-Glu 669

Attributs du produit: A DNA sequence encoding the human NCSTN (NP_056146.1) extracellular domain (Met 1-Glu 669) was expressed, with a polyhistidine tag at the C-terminus.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Nicastrin (NCSTN)

Autre désignation: Nicastrin/NCSTN

Sujet:

Background: Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.

Synonym: APH2,ATAG1874,KIAA0253,NCSTN,RP11-517F10.1

Poids moléculaire: 72.4 kDa

NCBI Accession: NP_056146

Pathways: Signalisation Notch, Neurotrophin Signaling Pathway