NOG Protein (Fc Tag)

N° du produit ABIN7317939

Cette protéine Recombinant NOG est produite dans HEK-293 Cells.

Aperçu rapide pour NOG Protein (Fc Tag) (ABIN7317939)

Antigène: NOG (Noggin (NOG))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette NOG protéine est marqué à la Fc Tag.

Fonction: Recombinant Human Noggin/NOG Protein (aa 1-232, Fc Tag)(Active)

Séquence: Met 1-Cys 232

Attributs du produit: A DNA sequence encoding the human Noggin precursor (NP_005441.1) (Met 1-Cys 232) was fused with the Fc region of human IgG1 at the C-terminus.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: 1. Measured by its ability to inhibit BMP2-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 1.5-2.0 μg /mL in the presence of 0.25-0.5 μg/mL of BMP-2.2. Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 0.1-0.6 μg/mL in the presence of 50 ng/mL of hBMP4.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: NOG (Noggin (NOG))

Autre désignation: Noggin/NOG

Sujet:

Background: Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities; with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs; Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7; and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes; including embryonic dorsal-ventral axis; induction of neural tissue; formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4; along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development; joint formation; and neural tube fusion.

Synonym: Noggin;SYM1;SYNS1

Poids moléculaire: 49.8 kDa

NCBI Accession: NP_005441

Pathways: Stem Cell Maintenance, Tube Formation