ERBB3 Protein (AA 730-1065) (His-GST)

N° du produit ABIN7197657

Cette protéine Recombinant ERBB3 est produite dans Baculovirus infected Insect Cells.

Aperçu rapide pour ERBB3 Protein (AA 730-1065) (His-GST) (ABIN7197657)

Antigène: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: Bio-Layer Interferometry (BLI)

Pureté: > 75 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 730-1065

Purification/Conjugué: Cette ERBB3 protéine est marqué à la His-GST.

Fonction: Recombinant Human HER3/ErbB3 Protein (aa 730-1065, His &GST Tag)

Séquence: Pro 730-Ser 1065

Attributs du produit: A DNA sequence encoding the cytoplasmic domain (Pro 730-Ser 1065) of human ErbB3 (NP_001973.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. No Kinase Activity 2. Using the Octet RED System, the affinity constant (Kd) of human ErBB3-GST bound to human NRG1 (aa 2-224) was 20 nM.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 7.5, 10 % glycerol, 1 mM GSH
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Autre désignation: HER3/ErbB3

Sujet: EEBB3,ErbB-3,HER3,LCCS2,MDA-BF-1,c-erbB-3,c-erbB3,erbB3-S,p180-ErbB3,p45-sErbB3,p85-sErbB3,ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Poids moléculaire:

Calculated MW: 65.0 kDa

Observed MW: 65 kDa

ID gène: 2065

NCBI Accession: NP_001973

UniProt: P21860

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway