SMAD3 Protein (AA 1-425) (His-GST)

N° du produit ABIN7320082

Cette protéine Recombinant SMAD3 est exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour SMAD3 Protein (AA 1-425) (His-GST) (ABIN7320082)

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Type de proteíne: Recombinant

Origine: Souris

Source: Baculovirus infected Insect Cells

Pureté: > 85 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 1-425

Purification/Conjugué: Cette SMAD3 protéine est marqué à la His-GST.

Fonction: Recombinant Mouse SMAD3 Protein (His & GST Tag)

Séquence: Met1-Ser425

Attributs du produit: A DNA sequence encoding the mouse SMAD3 (NP_058049.3) (Met1-Ser425) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus. Mouse, Human and Rat sequences are identical.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Autre désignation: SMAD3

Sujet: AU022421,Madh3,SMAD3 belongs to the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD3 is involved in cell signalling. It modulates signals of activin and TGFβ's. Binding of SMAD3 with SMAD4 enables its transmigration into the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD3 is a receptor-regulated SMAD (R-SMAD). In mice, mutation of SMAD3 has been linked to colorectal adenocarcinoma, increased systemic inflammation, and accelerated wound healing. Increased SMAD3 activity has been implicated in the pathogenesis of scleroderma. Smad3 is also a multifaceted regulator in adipose physiology and the pathogenesis of obesity and type 2 diabetes.

Poids moléculaire: 75.9 kDa

ID gène: 25631

UniProt: P84025

Pathways: Cycle Cellulaire, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy