UBE2H Protéine

N° du produit ABIN7198586

Protéine Recombinant UBE2H exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour UBE2H Protéine (ABIN7198586)

Antigène: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 93 % as determined by reducing SDS-PAGE.

Détail du produit

Fonction: Recombinant Human Ube2H Protein

Séquence: Met 1-Leu 183

Attributs du produit: A DNA sequence encoding the human UBE2H (P62256) (Met 1-Leu 183) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, 10 % glycerol, 2 mM DTT, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: UBE2H (Ubiquitin-Conjugating Enzyme E2H (UBE2H))

Autre désignation: Ube2H

Sujet:

Background: UBE2H is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. 

Synonym: Ubiquitin-Conjugating Enzyme E2 H; UbcH2; Ubiquitin Carrier Protein H; Ubiquitin-Conjugating Enzyme E2-20K; Ubiquitin-Protein Ligase H; UBE2H;E2-20K;GID3;UBC8;UBCH;UBCH2

UniProt: P62256