VEGFA Protein (AA 27-190)

N° du produit ABIN7320681

Cette protéine Recombinant VEGFA est exprimée dans Yeast (Pichia pastoris).

Aperçu rapide pour VEGFA Protein (AA 27-190) (ABIN7320681)

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Yeast (Pichia pastoris)

Application: Bio-Layer Interferometry (BLI)

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 27-190

Fonction: Recombinant Mouse VEGF-A/VEGF164 Protein

Séquence: Ala27-Arg190

Attributs du produit: Recombinant Mouse Vascular Endothelial Growth Factor A is produced by our Yeast expression system and the target gene encoding Ala27-Arg190 is expressed.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Loaded Mouse VEGFR2-Fc(PKSM041405) on Protein A Biosensor, can bind Mouse VEGF 164(CX73) with an affinity constant of 0.44 nM as determined in BLI assay.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Autre désignation: VEGF-A/VEGF164

Sujet: VEGF-A,VEGF164,VEGFA,VEGFA164,VPF,Vascular endothelial growth factor A,Vascular permeability factor,Mouse Vascular endothelial growth factor (VEGF or VEGF-A), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF/VEGF growth factor family that is characterized by a cystine knot structure formed by eight conserved cysteine residues. Alternately spliced isoforms of 120, 164 and 188 aa found in mouse. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-/KDR) on endothelial cells.Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells.It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

Poids moléculaire:

Calculated MW: 19.3 kDa

Observed MW: 18-22 kDa

ID gène: 22339

UniProt: Q00731

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling