CD25 Protein (AA 22-213) (Biotin,His-Avi Tag)

N° du produit ABIN7274200

Cette protéine Recombinant CD25 est exprimée dans HEK-293 Cells.

Aperçu rapide pour CD25 Protein (AA 22-213) (Biotin,His-Avi Tag) (ABIN7274200)

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 22-213

Purification/Conjugué: Cette CD25 protéine est marqué à la Biotin,His-Avi Tag.

Fonction: Biotinylated Human IL-2 R alpha/CD25 Protein

Séquence: Glu22-Cys213

Attributs du produit: Recombinant Biotinylated Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu22-Cys213.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human IL-2 R alpha, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 3.9 ng/ml determined by ELISA (QC Test).,Biotinylated Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: IL-2 R alpha

Sujet: Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Poids moléculaire: 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: P01589

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation